彩虹校准微球，Rainbow Calibration Particles,8 peaks, 1E7/mL, 3.0-3.4 um
SPHEROTM Calibration Particles are designed for routine calibration of flow cytometers. They are used extensively by many laboratories for QC and long term performance tracking of the flow cytometer. In addition, they are also used for routine alignment and calibration in fluorescence and confocal fluorescence microscopy.
The Rainbow Calibration Particles (RCPs) contain a mixture of several similar size particles with different fluorescence intensities. Every particle contains a mixture of fluorophores that allows excitation at any wavelength from 365 to 650 nm. As a result, most channels in the flow cytometer can be calibrated using the same set of particles.
These particles are also used to determine the relative voltage range for each flow cytometry detector. This will determine the dynamic range of specific PMT detectors.
The RCPs provide a reliable and reproducible particle mixture for calibrating flow cytometers. They are very stable since the fluorochromes are entrapped within the particles instead of being located on the surface. In addition, Spherotech uses fluorophores that are non-spectral matching to the commonly used fluorophores such as FITC, PE or PE-Cy5. As a result, the RCPs are stable in terms of fluorescence.