GeneSilencer®siRNA转染试剂

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GeneSilencer® siRNA Transfection Reagent is a novel cationic lipid formulation specifically designed for efficient delivery of siRNAs (small interfering RNAs) into a wide variety of cell types. siRNAs are short, gene-specific double-stranded RNAs that can cause gene silencing in mammalian cells by catalytically cleaving greater than 95% of the target mRNA (1,2,3).

 

Contents

Hydrated GeneSilencer Lipid

siRNA Diluent (corresponding ratio for each kit)

 

Storage

GeneSilencer siRNA Transfection Reagent is shipped at room temperature. For maximum stability, store all reagents at +4C upon receipt. If stored properly, all components are stable for 6 months.

 

GeneSilencer Transfection Reagent Kit ordering information

50 rxn kit, 0.18 ml, T500020

200 rxn kit, 0.75 ml, T500750

1000 rxn kit, 5 x 0.75 ml, T505750

10 rxn sample kit, 0.03 ml, T500020S

  

GeneSilencer Cell Line Citations: 3T3-L1, A549, A7, AGS, ASM Transformed, B16-F1, BEL7404, Bronchial C166, C2C12, C6R, CHO-K1, DC2.4, GS-KB-3-1, H22, H441, H460, H9c2, hCMEC/D3, HCP40, HCT-116, HEK 293, HEK 293T, HEK-293, HeLa, HepG2, HL-60, HMEC-1, HPAEC, HT29, HUT-78, JAR, Jurkat, KATOIII, L/Stab-2, LNCaP, M2, McA-RH 7777, MCF-7, MDA MB-231, MDA MB-468, MDA-MB-435, MG63, MKN 28, MKN 45, MV-4-11, Neuro2a, NIH-3T3, OK, PC-12, Cortical Neurons, Hippocampal Neurons, Macrophage, Pulmonary Artery, Endothelium, Dendritic Cells(Bone Marrow), Cortical Neurons, Coronary Artery Endothelial, Peritoneal Macrophage, Dorsal Root Ganglion Neurons, Retinal Ganglion, Dorsal Root Ganglion, Schwann Cells Cardiac Fibroblast, Cardiac Myocytes (Neonatal), Lung Microvascular Endothelial Cells, Aortic Muscle Cells, Dendritic Cells, Cerebellar Neurons, Osteoblast, HMVEC, Cardiac Myocytes, T-Cells (CD3+), Cortical Neurons, Dendritic Cells, Cortical Neurons, Aveolar Type II Cells, Macrophage, Dorsal Root Ganglion Lacrimal Gland Acinar Cells, Bone Marrow, Dendritic Cells, Pulmonary Artery Endothelium, Primary HPAEC. RAW 264.7, Renca, SaOs2, Saos-2, SCCH196, SNB19, SW480, U20S

  • High siRNA transfection efficiency
  • Functional gene silencing post siRNA delivery
  • Compatibility with diverse growth conditions (with and without serum)
  • Low cytotoxicity
  • Easy-to-use protocols for both adherent and suspension cells

References

Elbashir, S.M. et al. (2001) Nature 411: 494-498.

Caplen, N.J. et al. (2001) Proc Natl Acad Sci USA 98: 9742-9747.

Sharp, P.A. (2001) Genes and Development 15: 485-490.

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